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ACS plus organic acids have been reported to disable proton pumps in bacterial membranes and thus act as metabolic inhibitors. EPL on the other hand is adsorbed into the cell surface of microorganisms as a result of its cationic properties and strips the outer membrane and distributes the cytoplasm. LAE in comparison prevents bacterial growth by altering the cell membrane structure of microorganisms (35). Thus, sequential application of two of these decontamination agents in combination might result in greater pathogen reductions than any single application of these agents on beef carcass surfaces due to their different modes of action.
The stated objectives for this work were:
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