Project Summary

Prevalence, Quantity and Antibiotic Resistance Profiles of Salmonella enterica Late in the Feeding Period and at Slaughter in Response to Antibiotic use Early in the Cattle Feeding Period ‐ Part 2

Principle Investigator(s):
H. Morgan Scott1, Keri Norman1, Sara Lawhon1, Sam Ives2, Guy H. Loneragan3    
1Texas A&M University
2West Texas A&M University
3Texas Tech University 
Completion Date:
June 2017
The threat of antimicrobial resistant bacteria and the implications for human health represent an area of research that has been receiving a great deal of attention in both the media and scientific community. In the recent Centers for Disease Control and Prevention (CDC) Report, “Antibiotic Resistance Threats in the United States, 2013”, multidrug-resistant Salmonella enterica is listed as a microorganism of serious threat. Salmonella outbreaks are most commonly associated with poultry and eggs; however, beef has also been implicated as a source of infection. The use of antibiotics in food animals may contribute to selection and expansion of antimicrobial resistant bacteria through the food chain. A major concern is that many antibiotics used in food animals are also important in human medicine. Strict regulations or bans on the use of antimicrobials in agriculture could have devastating effects on animal health and create food safety issues. Identifying optimal ways to minimize any risks to human health associated with antibiotic use while maintaining animal health and welfare are important goals. 

The objective of this study was to determine the effects of tulathromycin and ceftiofur treatment on the prevalence, quantity, and antimicrobial resistance of Salmonella in feedlot cattle immediately following treatment, through the feeding period, and at slaughter. 
Individual fecal samples were collected at an experimental feedlot in Canyon, Texas. The randomized and controlled field trial was comprised of 134 steers in three different treatment groups to assess the impact of ceftiofur and tulathromycin use as metaphylaxis early in the feeding period. Twelve pens (11-12 steers/pen) were assembled with steers blocked by starting weights. Cattle fecal samples from Days 0, 7, 28, 56, and 99 then pre‐slaughter) were enriched and plated to selective media to identify Salmonella positive samples. Spiral plating onto brilliant green agar with novobiocin was used to obtain crude estimates of Salmonella quantities. Antibiotic susceptibility was performed for 14 different antimicrobials by the microbroth dilution method using the Sensititre® automated inoculation delivery and fluorometric plate reading system. Genotypic antimicrobial susceptibility profiles were determined by whole genome sequencing of Salmonella isolates. Sequencing data were analyzed for the presence of antimicrobial resistance genes using the online databases Resfinder, ARG‐ANNOT and CARD. Salmonella sequence types, virulence genes and plasmids were determined using whole genome sequencing data and short read sequence typing for bacterial pathogens (SRST2), while serotypes were classified at SeqSero. Hide swabs (1 m2) and subiliac lymph nodes were sampled at slaughter with similar analysis conducted as described for fecal samples. 
Use of antibiotics early in the feeding period has a limited impact on the ecology of Salmonella in the feces of cattle at later periods. Observed effects were transient, and the dominant serotypes present in the beef cattle environs represent a highly antibiotic susceptible population of Salmonella. The major effect on Salmonella prevalence and quantity appears to be climatic and may be limited to this region.
The dynamics of the Salmonella population in cattle during the feeding period and at slaughter has important food safety implications. This study suggests that by the time antibiotic treated cattle are sent to slaughter, fecal bacterial populations have returned to baseline conditions. The prevalence and quantity of Salmonella in hide swabs is nearly identical across all treatment groups at slaughter, suggesting a reversion to the local environment. There also is no significant impact of antibiotic use early in the feeding period on the quantity of Salmonella in lymph nodes at slaughter.