As the young and dynamic field of sustainability science continues to evolve, there is a need to adapt and update the methodologies used in life cycle and broader sustainability assessments of the beef industry. Consequently, this project updated and expanded the original Eco-efficiency Analysis to the SimaPro™ computational platform, providing the framework for open and transparent assessment of sustainability metrics for the beef industry, and enabling rapid updating of data as well as scenario testing in the future. This work also established the relative contribution of the beef production sector to the national and regional economies.