Project Summary

Understanding the Impact of the Presence of Relative Humidity in the Beef Cooking Process on Beef Tenderness, Sensory, Protein Stability, and Appearance

Principle Investigator(s):
D. Woerner, J. Martin, J.D. Tatum, R. Delmore, K. Belk and D. Gredell
Institution(s):
Colorado State University
Completion Date:
June 2016
Background

Recent Checkoff‐funded research has demonstrated that differences in steak thickness, cooking method, cooking temperature, cooking rate, and the inclusion of high levels of humidity influence the overall eating satisfaction of steaks and influences beef flavor (Shubert, 2014). Perhaps the most intriguing discovery of that research was the improvement in the tenderness observed in steaks that were cooked more slowly with 100% added humidity (Shubert, 2014). Additionally, pilot work (unpublished data) in the sensory lab at Colorado State University has demonstrated that increasing the added humidity in the cooking environment significantly increases the tenderness of beef steaks. This finding has led to speculation that the humidity in the cooking environment and/or equipment (e.g., open‐hearth grill or char broiler, belt grills, impingement ovens, clamshells, convection ovens) can influence the tenderness results in research trials depending on the type of equipment used to cook steaks and even the environment that the steaks are cooked in. It has been widely demonstrated that increased humidity (moisture) in the cooking process helps to degrade protein, specifically collagen, and thereby improves tenderness. This research is aimed at expanding upon the research idea that rate of cooking and the incorporation of humidity in the cooking environment can be major contributors to steak tenderness and sensory characteristics. Ultimately, the findings of this research will contribute to developing the ideal cooking procedures to maximize the steak eating experience and may also help to explain differences in research findings in previously conducted work related to beef tenderness. The objective of this study was to evaluate the effects of oven temperature and added humidity on the appearance, extent of protein denaturation, and palatability of beef strip loin steaks. The objectives were met by evaluating external and internal cooked steak color, extent of protein denaturation, total collagen, shear force, and using a trained sensory panel to characterize palatability traits.

Methodology

The study was designed as a 2 3 factorial representing two oven treatments [low temperature (LT) and high temperature (HT)] and three levels of added humidity [zero (ZH), mid (MH), and high (HH)], for a total of six individual treatments (Table 1). USDA Choice beef Strip Loins (n = 30) were used for this study. Each Strip Loin was fabricated into six sets of paired steaks, with each steak being 2.54 cm thick. Each set of paired steaks was randomly assigned into one of the treatments, so that each of the six treatments were represented within each individual Strip Loin. Individual steaks were vacuum‐packaged, aged for 14 days, then frozen until analysis. Prior to all analyses, steaks were cooked using the prescribed treatments to a final internal temperature of 71°C. Each sample was evaluated by a trained sensory panel to evaluate tenderness, juiciness, and flavor attributes of cooked steaks. Twelve samples were fed per session over 15 panels, each consisting of seven‐nine panelists. External and internal cooked color was measured using both instrumental readings (CIE L*a*b*) as well as subjective color evaluations by trained personnel. Differential scanning calorimetry (DSC) was used to measure the amount of energy required to denature any remaining intact proteins after the cooking process. Concentrations of total collagen were measured to evaluate cooking rate and humidity effects on the heat stability of connective tissue. Both slice shear force (SSF) and Warner‐Bratzler shear force (WBSF) were used as an objective measurement of tenderness.

Findings

The present study showed that adding humidity to the cooking environment significantly increased cooking rate, which was especially evident at a low oven temperature. When high levels (70‐100%) of humidity were added to the cooking environment, sensory panel ratings showed Strip steaks were tougher; however, when only mid-levels (35‐50%) of humidity were added at low oven temperature, steaks cooked considerably faster without toughening. Additionally, sensory panel ratings indicated that steaks cooked at lower oven temperatures were found to be more tender than when cooked at higher temperatures. Improvements in tenderness were the result of a slower cooking rate. Adding humidity to the cooking environment restricted surface browning of steaks and the development of desirable meat‐like flavors associated with these reactions. It is evident that humidity plays a significant role in sensory development and needs to be controlled in the cooking environment to maximize palatability and the overall eating experience. At both high and low oven temperatures, high levels of humidity were detrimental to palatability and should be avoided. However, at a low oven temperature, a mid-level (50%) of humidity-maintained tenderness and increased juiciness, while having minimal effects on flavor attributes.

Implications

The results of this study help to better understand the implications that oven temperature and added humidity have on beef steak sensory characteristics. It is clear that cooking method plays a significant role in sensory development; therefore, identifying cooking parameters to maximize eating potential is incredibly beneficial to the foodservice industry. As shown by trained sensory results, tenderness advantages of cooking at low oven temperatures can be achieved if juiciness is maintained by adding moderate levels of humidity to the cooking environment.

Table 1. Trained sensory ratings1 for beef LM steaks cooked to 71°C using two oven temperatures and three levels of humidity.

 

80°C

204°C

 

P – Value

Trait

Zero  Humidity

Mid

Humidity

High  Humidity

Zero  Humidity

Mid  Humidity

High  Humidity

SEM2

Oven Temp

Added Humidity

OT RH

Initial Tenderness

67.43am

67.17am

62.77an

62.84bm

63.63bm

60.19bn

1.62

< 0.01

< 0.01

0.44

Sustained

Tenderness

64.81am

63.41am

58.60an

59.27bm

59.61bm

54.98bn

1.51

< 0.01

< 0.01

0.47

Overall Tenderness

66.42am

65.53am

60.81an

61.51bm

61.44bm

57.55bn

1.54

< 0.01

< 0.01

0.61

Juiciness

41.04z

60.16w

56.42x

52.72y

52.02y

51.36y

2.09

0.43

< 0.01

< 0.01

Beefy/ Brothy

56.00w

50.45xy

49.32y

52.22x

51.24xy

50.37xy

2.68

0.37

< 0.01

< 0.01

Browned/Grilled

47.76m

42.34n

40.88o

47.03m

45.81n

42.52o

3.27

0.10

< 0.01

0.16

Buttery/ Fat

22.83y

26.07wx

23.82xy

27.93w

26.85w

22.83y

2.87

0.01

< 0.01

< 0.01

Burnt

6.90m

5.56n

7.09m

7.04m

4.31n

7.46m

1.28

0.72

< 0.01

0.57

Bloody/Metallic

10.31y

18.20w

15.79w

10.12y

12.82x

12.98x

2.07

< 0.01

< 0.01

0.01

Livery

0.79

0.23

0.39

0.16

0.14

0.63

0.22

0.29

0.18

0.07

Oxidized

0.82

0.69

0.47

0.76

0.91

0.84

0.38

0.31

0.75

0.60

  • a-bLeast square means in the same row without a common superscript differ (P < 0.05) due to oven temperature 
  • m-oLeast square means in the same row without a common superscript differ (P < 0.05) due to added humidity 
  • w-zLeast square means in the same row without a common superscript differ (P < 0.05) due to an over temperature relative humidity interaction 
  • 1Attributes were scored using a 100 mm unstructured line scale: 0 = very tough, very dry, and not present; 100 = very tender, very juicy, and very intense 
  • 2Standard error of the least squares means

References
  • Schubert, D. M., K. E. Belk, J. D. Tatum, R. J. Delmore, and A. Hess. 2014. Discovering Consumer Preferences for     Steak Thickness and Common Food Service Cookery Methods for Beef Strip Steaks. Colorado State       University.